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https://ahro.austin.org.au/austinjspui/handle/1/31835| Title: | Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry. | Austin Authors: | Pang, Lokman;Ernst, Matthias ;Huynh, Jennifer | Affiliation: | Olivia Newton-John Cancer Research Institute | Issue Date: | 2023 | Date: | 2022-12 | Publication information: | Methods in Molecular Biology 2023 | Abstract: | Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC staining workflow using specific antibodies against CD4, CD8α, FOXP3, and B220 to identify distinct lymphocyte populations including T and B cells. This staining strategy can be adapted to include other cell markers to evaluate the immune contexture in murine tissues. | URI: | https://ahro.austin.org.au/austinjspui/handle/1/31835 | DOI: | 10.1007/978-1-0716-2811-9_20 | ORCID: | Journal: | Methods in Molecular Biology | Start page: | 307 | End page: | 316 | PubMed URL: | 36513940 | ISSN: | 1940-6029 | Type: | Journal Article | Subjects: | Cell surface markers Immune cells Multiplex immunohistochemistry Spatial phenotyping Tyramide signal amplification (TSA) |
| Appears in Collections: | Journal articles |
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