Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/31835Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Pang, Lokman | - |
| dc.contributor.author | Ernst, Matthias | - |
| dc.contributor.author | Huynh, Jennifer | - |
| dc.date | 2022-12 | - |
| dc.date.accessioned | 2023-01-12T04:50:26Z | - |
| dc.date.available | 2023-01-12T04:50:26Z | - |
| dc.date.issued | 2023 | - |
| dc.identifier.citation | Methods in Molecular Biology 2023 | en_US |
| dc.identifier.issn | 1940-6029 | - |
| dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/31835 | - |
| dc.description.abstract | Multiplex immunohistochemistry (mIHC) facilitates the simultaneous detection of various immune cell markers on a single tissue section. Here, we describe a protocol for an mIHC staining workflow using specific antibodies against CD4, CD8α, FOXP3, and B220 to identify distinct lymphocyte populations including T and B cells. This staining strategy can be adapted to include other cell markers to evaluate the immune contexture in murine tissues. | en_US |
| dc.language.iso | eng | - |
| dc.subject | Cell surface markers | en_US |
| dc.subject | Immune cells | en_US |
| dc.subject | Multiplex immunohistochemistry | en_US |
| dc.subject | Spatial phenotyping | en_US |
| dc.subject | Tyramide signal amplification (TSA) | en_US |
| dc.title | Spatially Characterizing the Immune Contexture in Mouse Tissue Using Multiplex Immunohistochemistry. | en_US |
| dc.type | Journal Article | en_US |
| dc.identifier.journaltitle | Methods in Molecular Biology | en_US |
| dc.identifier.affiliation | Olivia Newton-John Cancer Research Institute | en_US |
| dc.identifier.doi | 10.1007/978-1-0716-2811-9_20 | en_US |
| dc.type.content | Text | en_US |
| dc.identifier.pubmedid | 36513940 | - |
| dc.description.volume | 2593 | - |
| dc.description.startpage | 307 | - |
| dc.description.endpage | 316 | - |
| local.name.researcher | Ernst, Matthias | |
| item.grantfulltext | none | - |
| item.openairetype | Journal Article | - |
| item.languageiso639-1 | en | - |
| item.fulltext | No Fulltext | - |
| item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
| item.cerifentitytype | Publications | - |
| crisitem.author.dept | Olivia Newton-John Cancer Research Institute | - |
| Appears in Collections: | Journal articles | |
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