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https://ahro.austin.org.au/austinjspui/handle/1/9160| Title: | Microtitre plate and assay buffer alter detection of antiphosphatidylethanolamine antibodies in lupus anticoagulant positive plasma. | Austin Authors: | Drouvalakis, K A;Buchanan, Russell R C | Affiliation: | The University of Melbourne, Department of Medicine, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australia drouvaka@leland.stanford.edu |
Issue Date: | 15-May-1999 | Publication information: | Thrombosis Research; 94(4): 205-12 | Abstract: | The ELISA for detection of anti-phosphatidylethanolamine antibodies in patients with lupus anticoagulant was studied. The influence of microtitre plate types, antigen concentration, antibody dilution, and buffer system was evaluated. Human albumin in the blocking buffer gave greater numbers of positive plasma samples than bovine serum. Comparison of simultaneous plasma and serum samples showed only minor variation in binding characteristics. | Gov't Doc #: | 10336235 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/9160 | Journal: | Thrombosis research | URL: | https://pubmed.ncbi.nlm.nih.gov/10336235 | Type: | Journal Article | Subjects: | Animals Antibodies.analysis Buffers Cattle Enzyme-Linked Immunosorbent Assay.instrumentation.standards Humans Lupus Coagulation Inhibitor.blood.immunology Phosphatidylethanolamines.immunology Sensitivity and Specificity |
| Appears in Collections: | Journal articles |
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