Please use this identifier to cite or link to this item:
https://ahro.austin.org.au/austinjspui/handle/1/11042
Title: | The effect of hyperbaric oxygen on apoptosis and proliferation in severe acute pancreatitis. | Austin Authors: | Koh, Shir Lin;Tan, Joon Win;Muralidharan, Vijayaragavan ;Christophi, Christopher | Affiliation: | Department of Surgery, University of Melbourne, Austin Health Melbourne, Victoria, Australia | Issue Date: | 1-Dec-2009 | Publication information: | Hpb : the Official Journal of the International Hepato Pancreato Biliary Association; 11(8): 629-37 | Abstract: | This paper investigates the significance of apoptosis in severe acute pancreatitis (SAP) and the possible modulating effects of hyperbaric oxygen (HBO).Wistar rats (250-350 g) were induced with SAP by biliopancreatic infusion of 4% sodium taurocholate. Rats were randomized for HBO treatment. Pancreatic tissue was stained for apoptosis with immunohistochemistry (anti-CASPASE-3 antibody and TUNEL), and histopathology haematoxylin and eosin (H&E). Acini were stained for proliferation with an anti-KI67 antibody. ImageProPlus was used to quantify apoptosis and proliferation in acinar cells. Statistical analysis was performed with two-independent-sample t-test or non-parametric Mann-Whitney test.In normal acini there is a low rate of apoptosis (0.165 +/- 0.157%, 0.181 +/- 0.168%, 0.130 +/- 0.298% in CASPASE-3, H&E and TUNEL, respectively) and proliferation (0.951 +/- 0.926%) (mean +/- standard deviation [SD]). When compared with normal, apoptosis (CASPASE-3: 1.28 +/- 1.12%, P= 0.008; 2.40 +/- 3.04%, P= 0.101; 1.23 +/- 0.87%, P= 0.091; H&E: 0.47 +/- 0.36%, P= 0.051; 0.69 +/- 0.63%, P= 0.001; 0.68 +/- 0.28%, P= 0; TUNEL: 1.08 +/- 1.42%, P= 0; 1.96 +/- 1.87%, P= 0; 2.36 +/- 2.26%, P= 0) and proliferation (1.96 +/- 1.89%, P= 0.187; 1.73 +/- 1.76%, P= 0.165; 1.36 +/- 1.40%, P= 0.571) were increased on days 1, 2 and 3 post-induction, respectively. In comparison with the untreated controls, HBO increased apoptosis on day 1 (CASPASE-3: 3.11 +/- 1.97%, P= 0.04; H&E: 0.97 +/- 0.76%, P= 0.005) and day 2 (TUNEL: 3.61 +/- 3.05%, P= 0.034). Treatment with HBO increased proliferation (3.04 +/- 3.14%, P= 0.519; 7.33 +/- 7.55%, P= 0.153) on days 2 and 3, respectively, compared with the untreated controls.During SAP, acini apoptosis and proliferation were increased. Hyperbaric oxygen therapy may improve the condition of SAP by promoting apoptosis and proliferation. | Gov't Doc #: | 20495630 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/11042 | DOI: | 10.1111/j.1477-2574.2009.00099.x | Journal: | HPB : the official journal of the International Hepato Pancreato Biliary Association | URL: | https://pubmed.ncbi.nlm.nih.gov/20495630 | Type: | Journal Article |
Appears in Collections: | Journal articles |
Show full item record
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.