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https://ahro.austin.org.au/austinjspui/handle/1/10935
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DC Field | Value | Language |
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dc.contributor.author | Testro, Adam G | - |
dc.contributor.author | Gow, Paul J | - |
dc.contributor.author | Angus, Peter W | - |
dc.contributor.author | Wongseelashote, Sarah | - |
dc.contributor.author | Skinner, Narelle | - |
dc.contributor.author | Markovska, Vesna | - |
dc.contributor.author | Visvanathan, Kumar | - |
dc.date.accessioned | 2015-05-16T00:30:46Z | - |
dc.date.available | 2015-05-16T00:30:46Z | - |
dc.date.issued | 2009-12-16 | - |
dc.identifier.citation | Journal of Hepatology 2009; 52(2): 199-205 | en_US |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/10935 | en |
dc.description.abstract | Toll-like receptors (TLRs) are critical to innate immune responses. TLR4 recognises Gram-negative bacteria, whilst TLR2 recognises Gram-positive. We examined TLR expression and function in cirrhosis, and whether this is affected by antibiotic therapy.Sixty-four subjects were included (23 controls and 41 Child-Pugh C cirrhotic patients). Thirty patients were taking norfloxacin or trimethoprim-sulfamethoxazole as prophylaxis against bacterial peritonitis and 11 were not. In a second study, 8 patients were examined before and after commencement of antibiotics. Monocyte expression of TLR2 and 4 was determined by flow cytometry. Monocytes from the patients with paired samples were stimulated using TLR ligands and TNF-alpha production measured.Patients not taking antibiotics had significantly decreased TLR4 expression compared with controls (0.74 vs. 1.0, p=0.009) and patients receiving antibiotics (0.74 vs. 0.98, p=0.02). There were no differences with regard to TLR2. In the patients with paired samples, TLR4 expression increased (0.74-1.49, p=0.002) following antibiotic use, whilst again, there was no change in TLR2 expression (0.99 vs. 0.92, p=0.20). TLR4-dependent TNF-alpha production increased following antibiotic use (1077 vs. 3620pg/mL, p<0.05), whilst TLR2-dependent production was unchanged.TLR4 expression is decreased in patients with Child-Pugh C cirrhosis, but is restored by antibiotics targeting enteric Gram-negative bacteria. TLR4-dependent cytokine production also increases significantly following antibiotic therapy. This suggests that the high incidence of Gram-negative infection in cirrhotic patients is in part due to down-regulation of the TLR4-dependant immune response and that the efficacy of antibiotic prophylaxis is contributed to by modulation of innate immunity. | en_US |
dc.language.iso | en | en |
dc.subject.other | Adult | en |
dc.subject.other | Aged | en |
dc.subject.other | Anti-Bacterial Agents.pharmacology | en |
dc.subject.other | Case-Control Studies | en |
dc.subject.other | Female | en |
dc.subject.other | Gram-Negative Bacterial Infections.prevention & control | en |
dc.subject.other | Humans | en |
dc.subject.other | Immunity, Innate.drug effects | en |
dc.subject.other | In Vitro Techniques | en |
dc.subject.other | Leukocytes, Mononuclear.drug effects.immunology | en |
dc.subject.other | Liver Cirrhosis.drug therapy.immunology | en |
dc.subject.other | Male | en |
dc.subject.other | Middle Aged | en |
dc.subject.other | Norfloxacin.pharmacology | en |
dc.subject.other | Peritonitis.prevention & control | en |
dc.subject.other | Toll-Like Receptor 2.metabolism | en |
dc.subject.other | Toll-Like Receptor 4.metabolism | en |
dc.subject.other | Trimethoprim-Sulfamethoxazole Combination.pharmacology | en |
dc.subject.other | Tumor Necrosis Factor-alpha.biosynthesis | en |
dc.title | Effects of antibiotics on expression and function of Toll-like receptors 2 and 4 on mononuclear cells in patients with advanced cirrhosis. | en_US |
dc.type | Journal Article | en_US |
dc.identifier.journaltitle | Journal of Hepatology | en_US |
dc.identifier.affiliation | Medicine (University of Melbourne) | en_US |
dc.identifier.doi | 10.1016/j.jhep.2009.11.006 | en_US |
dc.description.pages | 199-205 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/20006396 | en |
dc.type.content | Text | en_US |
dc.type.austin | Journal Article | en |
local.name.researcher | Angus, Peter W | |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.openairetype | Journal Article | - |
item.fulltext | No Fulltext | - |
crisitem.author.dept | Victorian Liver Transplant Unit | - |
crisitem.author.dept | Gastroenterology and Hepatology | - |
crisitem.author.dept | Victorian Liver Transplant Unit | - |
crisitem.author.dept | Gastroenterology and Hepatology | - |
crisitem.author.dept | Victorian Liver Transplant Unit | - |
crisitem.author.dept | Gastroenterology and Hepatology | - |
Appears in Collections: | Journal articles |
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