Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10774
Title: Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research.
Austin Authors: Lubel, J S;Herath, C B;Velkoska, Elena;Casley, David J;Burrell, Louise M ;Angus, Peter W 
Affiliation: General Medicine
Issue Date: 18-Feb-2009
Publication information: Cytotechnology 2009; 58(3): 119-26
Abstract: Cell culture experiments often employ the use of culture media that contain fetal calf serum (FCS). The angiotensin peptides angiotensin II and angiotensin 1-7 have opposing effects with angiotensin converting enzyme 2 (ACE2) being the enzyme predominantly responsible for generating angiotensin 1-7 from angiotensin II. The effect of FCS on angiotensin peptides has not previously been described. We have shown that FCS has ACE2 enzyme activity capable of degrading angiotensin II and generating angiotensin 1-7. Researchers should be aware that FCS possesses ACE2 activity and that heat-treating FCS to 56 degrees C only partially inhibits this enzyme activity, whereas heat-treating to 70 degrees C completely abolishes ACE2 activity.
URI: https://ahro.austin.org.au/austinjspui/handle/1/10774
DOI: 10.1007/s10616-009-9185-0
ORCID: 
Journal: Cytotechnology
URL: https://pubmed.ncbi.nlm.nih.gov/19224387
Type: Journal Article
Appears in Collections:Journal articles

Files in This Item:
File SizeFormat 
19224387.pdf51.9 kBAdobe PDFView/Open
Show full item record

Page view(s)

42
checked on Nov 18, 2024

Download(s)

88
checked on Nov 18, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.